Solution：

Enzymatic activity in enzyme assays include:

Reduction method: the enzyme and the substrate react under specific conditions, adding a chemical reagent in the reaction system, and the product of the enzymatic reaction reacts with the chemical reagent to generate a colored substance. By colorimetric at a specific wavelength, the amount of the reduced product was determined to calculate the magnitude of the enzyme activity.

Color Protoplasm Method: Synthesized artificial substrate by binding substrate to specific soluble chromophore species. The substrate reacts with the enzyme to release the chromophore, and the color depth is determined spectrophotometrically. After comparing with the curves of known standard enzymes, the activity of the enzyme to be tested can be obtained.

Viscosity method: This method is often used to determine the activity of cellulase, xylanase and β-glucanase. When the enzyme on the viscous substrate will make its viscosity is greatly reduced.

Determination of kinematic viscosity of the solvent and the sample solution under certain conditions, we can calculate the intrinsic viscosity, and in order to determine the enzyme activity.

High Performance Liquid Chromatography: After the enzyme and its substrate are fully reacted under specific conditions, the solution is extracted from the reaction system for chromatographic analysis, the peak height and the half-height of each sample are measured, and the content of the enzymatic reaction product is calculated, Thus converting the value of enzyme activity.

Immunological methods: According to the enzyme and its antibodies can occur between the specific precipitation reaction, enzyme and standard enzyme by comparison, the final determination of enzyme activity.

Agar gel diffusion method: the enzyme substrate and agar mixed melting, made of agar plate. Use a hole punch to punch a hole about 4-5 mm in radius on the agar plate. At the point of enzyme-like and cultured for 24h later, the stain was developed with a developing agent or a developing solvent to reveal the hydrolysis zone, and the enzyme activity was determined by the relationship between the hydrolytic diameter and the enzyme activity.